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KMID : 0382619880080020921
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Hanyang Journal of Medicine
1988 Volume.8 No. 2 p.921 ~ p.929
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A Study on the Detection of Neuroblastoma Cells from Bone Marrow Using Monoclonal Antibodies and Immunobeads
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Abstract
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For the detection of neuroblastoma cells from bone marrow, the author used four monoclonal antibodies(459, 390, HSAN 1.2 and RB 21-7) specific for neu-roblastoma cells and immunobeads(Dynabeads).
Two clinically suspected neuroblastoma patients were studied, and as study controls one healthy child :with: acute lymphoblastic leukemia. and those -two pa-tients without adding monoclonal antibodies were used.
The following results were obtained:
Cells coated with five or more beads were not observed in the control groups.
Cells coated with one to two beads observed in two patients were not signifi-cant, compared to the control groups.
Cells coated with three to four beads observed in two patients were statisti-cally significant, compared to the control groups(p<0.01 and p<0.05).
Cells coated with five or more beads were observed only in two patients´ ce-lls treated with monoclonal antibodies and immunobeads.
There were no differences according to the amount of monoclonal antibodies (15?g and 30?g/5 X 105 cells) and the number of immunobeads (2X106 and 3 X 106/5 X 105 cells).
Neuroblast-like cells were observed from the fourth day of bone marrow cell culture in Leibovitz´s L-15 (with 17% Fetal Bovine Serum) media of the two patients. This finding confirmed that the observed cells coated with´ immuno-beads were neuroblastoma cells.
This diagnostic procedure is a relatively simple and rapid, and may be of va-lue for the diagnosis of neuroblastoma metastasis to the bone marrow.
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